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مقایسه میزان آلودگی به مایکوپلاسما آگالاکتیه(Mycoplasma agalactiae) در گوسفندان و بزهای مبتلا به آگالاکسی واگیردار در استان کرمان

نویسنده:

علمی-پژوهشی (وزارت علوم) (6 صفحه - از 1001 تا 1006)

ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ ﺑﻌﻨﻮان ﻋﺎﻣﻞ آﮔﺎﻻﮐﺴﯽ واﮔﯿﺮدار در ﮔﻮﺳﻔﻨﺪ و ﺑﺰ ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﺳﺖ. اﯾﻦ ﺑﯿﻤﺎری در ﺑﺴﯿﺎری از ﻧﻘﺎط ﺟﻬﺎن وﺟﻮد دارد و در اﯾﺮان ﻧﯿﺰ ﮔﺰارش ﮔﺮدﯾﺪه اﺳﺖ. ﻫﺪف از اﯾﻦ ﺗﺤﻘﯿﻖ ﻣﻘﺎﯾﺴﻪ ﻣﯿﺰان آﻟﻮدﮔﯽ ﺑﻪ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ در ﮔﻮﺳﻔﻨﺪان و ﺑﺰﻫﺎی ﻣﺒﺘﻼ ﺑﻪ آﮔﺎﻻﮐﺴﯽ واﮔﯿﺮدار ﺑﺎ اﺳﺘﻔﺎده از روش واﮐﻨﺶ زﻧﺠﯿﺮهای ﭘﻠﯿﻤﺮاز )PCR( در اﺳﺘﺎن ﮐﺮﻣﺎن ﺑﻮد. اﯾﻦ ﻣﻄﺎﻟﻌﻪ ﻣﻘﺎﯾﺴﻪ ای ﺑﻪ ﻣﺪت 6 ﻣﺎه ﺑﺮ روی ﻧﻤﻮﻧﻪ ﻫﺎی درﯾﺎﻓﺘﯽ ﻣﺸﮑﻮک ﺑﻪ آﮔﺎﻻﮐﺴﯽ واﮔﯿﺮدار در ﮔﻮﺳﻔﻨﺪان و ﺑﺰﻫﺎی اﺳﺘﺎن ﮐﺮﻣﺎن اﻧﺠﺎم ﮔﺮﻓﺖ. ﻧﻤﻮﻧﻪﻫﺎی درﯾﺎﻓﺘﯽ ﺷﺎﻣﻞ ﺗﺮﺷﺤﺎت ﻣﻠﺘﺤﻤﻪ ﭼﺸﻢ، ﺷﯿﺮ و ﻣﺎﯾﻊ ﻣﻔﺼﻠﯽ ﺑﻮد. ﺟﻨﺲ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ ﺑﺎ اﺳﺘﻔﺎده از آﻏﺎزﮔﺮﻫﺎی اﺧﺘﺼﺎﺻﯽ در ﻧﺎﺣﯿﻪ ژن 16S rRNA ﺑﺎ روش PCR و ﺗﺸﮑﯿﻞ ﺑﺎﻧﺪ 163 ﺟﻔﺖ ﺑﺎز در ﻧﻤﻮﻧﻪ ﻫﺎی درﯾﺎﻓﺘﯽ ﺷﻨﺎﺳﺎﯾﯽ ﮔﺮدﯾﺪ. ﺳﭙﺲ ﺗﻤﺎﻣﯽ ﻧﻤﻮﻧﻪﻫﺎی ﻣﺜﺒﺖ ﺑﺎ اﺳﺘﻔﺎده از آﻏﺎزﮔﺮﻫﺎی اﺧﺘﺼﺎﺻﯽ در ﻧﺎﺣﯿﻪ ﻟﯿﭙﻮ ﭘﺮوﺗﺌﯿﻦ ﺳﻄﺤﯽ ﺑﺎ ﺗﺸﮑﯿﻞ ﺑﺎﻧﺪ 375 ﺟﻔﺖ ﺑﺎز ﺟﻬﺖ ردﯾﺎﺑﯽ ﮔﻮﻧﻪ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ ﻣﻮرد ارزﯾﺎﺑﯽ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. از ﻣﺠﻤﻮع 142 ﻧﻤﻮﻧﻪ ﮔﻮﺳﻔﻨﺪ و 85 ﻧﻤﻮﻧﻪ ﺑﺰ، ﺗﻌﺪاد 41)59%( ﻧﻤﻮﻧﻪ ﮔﻮﺳﻔﻨﺪان و ﺗﻌﺪاد 54)46%( ﻧﻤﻮﻧﻪ ﺑﺰﻫﺎ از ﻧﻈﺮ ﺟﻨﺲ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ ﻣﺜﺒﺖ ﺑﻮدﻧﺪ . ﻫﻤﭽﻨﯿﻦ ﺗﻌﺪاد 29)17%( ﻧﻤﻮﻧﻪ از ﮔﻮﺳﻔﻨﺪان و ﺗﻌﺪاد 61)28%( ﻧﻤﻮﻧﻪ از ﺑﺰﻫﺎ ﺑﻪ ﮔﻮﻧﻪ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ آﻟﻮدﮔﯽ داﺷﺘﻨﺪ. ﻧﺘﺎﯾﺞ اﯾﻦ ﭘﮋوﻫﺶ ﻧﺸﺎن ﻣﯽ دﻫﺪ ﮐﻪ آﻟﻮدﮔﯽ ﺑﻪ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ در ﺑﺰﻫﺎ ﺑﯿﺸﺘﺮ از ﮔﻮﺳﻔﻨﺪان اﺳﺖ. ﺑﻨﺎﺑﺮاﯾﻦ ﻣﺎﯾﮑﻮﭘﻼﺳﻤﺎ آﮔﺎﻻﮐﺘﯿﻪ ﯾﮑﯽ از ﻋﻮاﻣﻞ اﺻﻠﯽ ﺑﯿﻤﺎری آﮔﺎﻻﮐﺴﯽ واﮔﯿﺮدار ﮔﻮﺳﻔﻨﺪ و ﺑﺰ در اﺳﺘﺎن ﮐﺮﻣﺎن ﻣﯽ ﺑﺎﺷﺪ.

Mycoplasma agalactiae is considered to be the classic agent of contagious agalactiae in sheep and goats. This disease is particularly widespread around the word. Iran is one of the countries that contagious agalactia is present. The aim of this study was to compare the infection rate of M. agalactiae in affected sheep with goats to contagious agalactia by Polymerase Chain Reaction (PCR) method in Kerman province. This comparative study was performed on all sheep and goats received suspected samples within the periods of 6 months in Kerman province. A total of 142 sheep and 85 goat samples were collected from conjunctival excretion، milk secretion and joint exudates. Mycoplasma genus was detected from samples، using specific primers of 16S rRNA gene with specific band of 163 bp، by PCR method. All mycoplasmapositive samples were investigated، using specific primers of surface lipoprotein with specific band of 375 bp، for M. agalactiae infection. Out of 142 samples، 59 (41%) sheep samples and 46 (54%) goats’ samples were positive with mycoplasma genus. Also 17 (29%) sheep samples and 28 (61%) goats’ samples were infected with Mycoplasma agalactiae. The results were shown that the infection with Mycoplasma agalactiae in goats was more than sheep. In conclusion، Mycoplasma agalactiae was the main agent of contagious agalactiae in sheep and goats in Kerman province.


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